Journal: British journal of cancer

Article Title: Inactivation of the WASF3 gene in prostate cancer cells leads to suppression of tumorigenicity and metastases.

doi: 10.1038/sj.bjc.6605850

Figure Lengend Snippet: Figure 7 Knockdown of WASF3 expression suppresses MMP9 expression levels and inhibits MMP9 release from prostate cancer cell lines. (A) RT–PCR analysis of PC3 and DU145 cells showing knockdown of WASF3 also show down-regulation of MMP9, but not MMP2 expression levels. Cont ¼ parental cells. (B) Secretion of the pro- and active form of MMP9 from PC3 and DU145 cells were measured in the supernatants of actively growing cells using the Fluorokine MAP assay procedure. Cells from each clone showing knockdown of WASF3 also showed reduced MMP9 levels compared with parental (control) and non-knockdown cells that did not.

Article Snippet: After 24 h, the media were collected in tubes and centrifuged for 10 min at 10 000 g. The pro- and active MMP-9 levels released into the media were measured using a Fluorokine MAP human MMP9 kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Knockdown, Expressing, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Tamarindus indica Seed Extract-Based Botanical Compositions Alleviate Knee Pain and Improve Joint Function in Mild-to-Moderate Osteoarthritis: A Randomized, Double-Blind, Placebo-Controlled Clinical Study

doi: 10.1155/2022/2226139

Figure Lengend Snippet: CONSORT diagram shows the flow of the trial process. Knee pain, musculoskeletal functions, and serum hsCRP were assessed at baseline and days 5, 28, and 56 of the study. Serum TNF- α , IL-6, MMP3, and urinary C-terminal cross-linked telopeptide of type II collagen (uCTX-II) were measured at baseline and end of the study.

Article Snippet: The TNF- α (Cat# DTA00D) and MMP3 (Cat# MMP300) ELISA kits were purchased from R&D Systems (Minneapolis, MN).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Tamarindus indica Seed Extract-Based Botanical Compositions Alleviate Knee Pain and Improve Joint Function in Mild-to-Moderate Osteoarthritis: A Randomized, Double-Blind, Placebo-Controlled Clinical Study

doi: 10.1155/2022/2226139

Figure Lengend Snippet: NXT15906F6 and NXT19185 supplementation improved serum markers in the study participants. (a–d) Mean ± SD of serum TNF- α ( n = 27), IL-6 ( n = 23), hsCRP ( n = 27), and MMP3 ( n = 27) levels, respectively. ∗ and $ indicate significance ( P < 0.05) in “within the group” (vs. baseline) and “between the groups” (vs. placebo) comparison analysis, respectively, using unequal variance t -test.

Article Snippet: The TNF- α (Cat# DTA00D) and MMP3 (Cat# MMP300) ELISA kits were purchased from R&D Systems (Minneapolis, MN).

Techniques: Comparison

Journal: The FASEB Journal

Article Title: Suppression of cigarette smoke induced MMP1 expression by selective serotonin re‐uptake inhibitors

doi: 10.1096/fj.202001966RR

Figure Lengend Snippet: Validation of and NMLSMR targets identified by the high throughput assay. A, To test for reproducibility of the assay, 293T cells were transfected with the MMP‐1/pGL3 reporter plasmid. Cells were seeded in three 96‐well plates using an interleaved format and treated with 5% CSE media (high‐signal) 2% CSE (medium‐signal) no CSE (low‐signal). After a 24‐h incubation, the luciferase activity was measured in each well. Representative data from days 1–3 is shown. The calculated Z' factor was 0.71 ± 0.05 SEM, n = 5. B, As expected, the ERK inhibitor PD98059 inhibited luciferase expression in a dose‐dependent fashion in positive controls exposed to 5% CSE ( *** p ≤ 0.001, significantly different from control; ### p ≤ 0.001, significantly different from promoter + CSE; ## p ≤0.01, significantly different from promoter + CSE). C, Data from the high throughout screen displaying each compound tested as a block dot. The y‐axis illustrates the change in MMP1 expression compared with expression levels in CSE exposed in HEK293 cells. The drugs in the −100% range (red shaded area) are capable of nullifying all CSE induced MMP1 transcriptional activity

Article Snippet: MMP‐1 Fluorokine E kits (R&D Systems F1M00) were used to determine MMP‐1 levels and performed per manufacturer's protocol.

Techniques: Biomarker Discovery, High Throughput Screening Assay, Transfection, Plasmid Preparation, Incubation, Luciferase, Activity Assay, Expressing, Control, Blocking Assay